Anti-NME1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-NME1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-NME1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-NME1 Magnetic Beads-IP Kit Product Description
The Anti-NME1 magnetic Beads, conjugated with Anti-NME1 antibody, are used for immuneprecipitation (IP) of NME1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing NME1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound NME1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-NME1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human NME1 protein (Catalog#11615-H07E)
Species Reactivity
Human NME1 / NDPKA
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human NME1 (rh NME1; Catalog#11615-H07E; NP_000260.1; Ala 2-Glu 152). NME1 IgG was purified by human NME1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-NME1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AWDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GAADALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NBSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NDKAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NDPK-AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NDPKAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NM23ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NM23-H1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
NME1 Background Information
NME1, also known as Nucleoside Diphosphate Kinase A (NDK-A), or NM23-H1, belongs to the NDK family. NM23-H1 is known to have a metastasis suppressive activity in many tumor cells. Recent studies have shown that the interacting proteins with NM23-H1 which mediate the cell proliferation, may act as modulators of the metastasis suppressor activity. The interacting proteins with NM23-H1 can be classified into 3 groups. The first group of proteins can be classified as upstream kinases of NM23-H1 such as CKI and Aurora-A/STK15. The second group of proteins acts as downstream effectors for the regulation of specific gene transcriptions, GTP-binding protein functions, and signal transduction in Erk signal cascade. The third group of proteins can be classified as bi-directionally influencing binding partners of NM23-H1. As a result, the interactions with NM23-H1 and binding partners have implications in the biochemical characterization involved in metastasis and tumorigenesis. NDKA is increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke. Additionally, NM23-H1 significantly reduces metastasis without effects on primary tumor size and was the first discovered metastasis suppressor gene.
Full Name
NME/NM23 nucleoside diphosphate kinase 1
References
Allard L, et al. (2005) PARK7 and nucleoside diphosphate kinase A as plasma markers for the early diagnosis of stroke. Clin Chem. 51(11): 2043-51. Steeg PS, et al. (2008) Clinical-translational approaches to the Nm23-H1 metastasis suppressor. Clin Cancer Res. 14(16): 5006-12. Kim HD, et al. (2009) Regulators affecting the metastasis suppressor activity of Nm23-H1. Mol Cell Biochem. 329(1-2): 167-73.
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