Anti-ALDH7A1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ALDH7A1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ALDH7A1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ALDH7A1 Magnetic Beads-IP Kit Product Description
The Anti-ALDH7A1 magnetic Beads, conjugated with Anti-ALDH7A1 antibody, are used for immuneprecipitation (IP) of ALDH7A1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ALDH7A1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ALDH7A1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-ALDH7A1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ALDH7A1 protein (Catalog#11614-H07E)
Species Reactivity
Human ALDH7A1
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH7A1 (rh ALDH7A1; Catalog#11614-H07E; P49419-2; Ser 2-Gln 511).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ALDH7A1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ATQ1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EPDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PDEALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ALDH7A1 Background Information
ALDH7A1 (Aldehyde dehydrogenase 7 family, member A1) is a member of subfamily 7 in the aldehyde dehydrogenase family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. Mammalian ALDH7A1 is homologous to plant ALDH7B1 which protects against various forms of stress such as increased salinity, dehydration and treatment with oxidants or pesticides. In mammals, ALDH7A1 is known to play a primary role during lysine catabolism through the NAD+-dependent oxidative conversion of aminoadipate semialdehyde (AASA) to its corresponding carboxylic acid, α-aminoadipic acid. Deleterious mutations in human ALDH7A1 are responsible for pyridoxine-dependent and folinic acid-responsive seizures. ALDH7A1 is a novel aldehyde dehydrogenase expressed in multiple subcellular compartments that protects against hyperosmotic stress by generating osmolytes and metabolizing toxic aldehydes.
Full Name
aldehyde dehydrogenase 7 family, member A1
References
Brocker C, et al. (2011) Aldehyde dehydrogenase 7A1 (ALDH7A1) attenuates reactive aldehyde and oxidative stress induced cytotoxicity. Chem Biol Interact. 191(1-3): 269-77. Brocker C, et al. (2010) Aldehyde dehydrogenase 7A1 (ALDH7A1) is a novel enzyme involved in cellular defense against hyperosmotic stress. J Biol Chem. 285(24): 18452-63.
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