Anti-SIRP alpha Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SIRP alpha Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SIRP alpha magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SIRP alpha Magnetic Beads-IP Kit Product Description
The Anti-SIRP alpha magnetic Beads, conjugated with Anti-SIRP alpha antibody, are used for immuneprecipitation (IP) of SIRP alpha proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SIRP alpha proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SIRP alpha proteins are dissociated from the magnetic beads using an elution buffer. Anti-SIRP alpha Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human SIRPA protein (Catalog#11612-H08H)
Species Reactivity
Human SIRPA / CD172A
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human SIRPA (rh SIRPA; Catalog#11612-H08H; NP_542970.1; Met 1-Arg 370). SIRPA specific IgG was purified by human SIRPA affinity chromatography .
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SIRP alpha Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-BITALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD172AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MFRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MYD-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-P84ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PTPNS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SHPS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SIRPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SIRP alphaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SIRP alpha Background Information
Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.
Immune Checkpoint Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy Full Name
signal-regulatory protein alpha
References
Timms JF. et al., 1999, Curr Biol. 9: 927-30. Stofega MR. et al., 2000, J Biol Chem. 275: 28222-9. Liu T. et al., 2005, J Proteome Res. 4: 2070-80. Wolf-Yadlin A. et al., 2007, Proc Natl Acad Sci. 104: 5860-5.
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