Anti-ST6GAL1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ST6GAL1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ST6GAL1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ST6GAL1 Magnetic Beads-IP Kit Product Description
The Anti-ST6GAL1 magnetic Beads, conjugated with Anti-ST6GAL1 antibody, are used for immuneprecipitation (IP) of ST6GAL1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ST6GAL1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ST6GAL1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-ST6GAL1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ST6GAL1 / ST6GAL-I protein (Catalog#11590-H07H)
Species Reactivity
Human ST6GAL1 / ST6GAL-I
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ST6GAL1 / ST6GAL-I (rh ST6GAL1 / ST6GAL-I; Catalog#11590-H07H; P15907; Lys 27-Cys406). ST6GAL1 / ST6GAL-I specific IgG was purified by Human ST6GAL1 / ST6GAL-I affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ST6GAL1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-SIAT1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ST6GalIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ST6NALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ST6GAL1 Background Information
Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein which belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the?Golgi?but which can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
Full Name
ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
References
Collins,B.E. et al., 2006, Nat Immunol. 7(2):199-206. Videira,P.A. et al., 2008, Glycoconj J. 25(3): 259-68. Petit,D. et al., 2010, J Biol Chem. 285(49): 38399-414. Kroes,R.A. et al., 2010, Proc Natl Acad Sci USA.107(28):12646-51.
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