Anti-Arginase Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-Arginase Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Arginase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Arginase Magnetic Beads-IP Kit Product Description
The Anti-Arginase magnetic Beads, conjugated with Anti-Arginase antibody, are used for immuneprecipitation (IP) of Arginase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Arginase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Arginase proteins are dissociated from the magnetic beads using an elution buffer. Anti-Arginase Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ARG1 / Arginase 1 protein (Catalog#11558-H08H)
Species Reactivity
Human ARG1 / Arginase 1
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ARG1 / Arginase 1 (rh ARG1 / Arginase 1; Catalog#11558-H08H; P05089-1; Met 1-Lys 322).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Arginase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ARG1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Arginase Background Information
Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.
References
Durante W, et al. (2007) Arginase: a critical regulator of nitric oxide synthesis and vascular function. Clin Exp Pharmacol Physiol. 34(9): 906-11. Waddington SN. (2002) Arginase in glomerulonephritis. Kidney Int. 61(3): 876-81. Morris SM. (2002). Regulation of enzymes of the urea cycle and arginine metabolism. Annual review of nutrition. 22 (1): 87-105.