Anti-CDC42 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CDC42 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CDC42 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CDC42 Magnetic Beads-IP Kit Product Description
The Anti-CDC42 magnetic Beads, conjugated with Anti-CDC42 antibody, are used for immuneprecipitation (IP) of CDC42 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CDC42 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CDC42 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CDC42 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CDC42 protein (Catalog#10062-H09E)
Species Reactivity
Human CDC42
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CDC42 (rh CDC42; Catalog#10062-H09E; P60953-2; Met1-Cys188). CDC42 specific IgG was purified by Human CDC42 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CDC42 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CDC42HsALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-G25KALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CDC42 Background Information
Full Name
cell division cycle 42
References
et al.
Shinjo K, et al. (1990) Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42. Proc Natl Acad Sci. 87(24):9853-7. Munemitsu S, et al. (1990) Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42. Mol Cell Biol. 10(11):5977-82. Walker S J, et al. (2000) Activation of phospholipase D1 by Cdc42 requires the Rho insert region. J Biol Chem. 275(21):15665-8. Low B C, et al. (1999) Tyrosine phosphorylation of the Bcl-2-associated protein BNIP-2 by fibroblast growth factor receptor-1 prevents its binding to Cdc42GAP and Cdc42. J Biol Chem. 274(46): 33123-30. Zhang B, et al. (1998) Interaction of Rac1 with GTPase-activating proteins and putative effectors. A comparison with Cdc42 and RhoA. J Biol Chem. 273(15):8776-82.
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