Anti-Mannan Binding Lectin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Mannan Binding Lectin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Mannan Binding Lectin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Mannan Binding Lectin Magnetic Beads-IP Kit Product Description
The Anti-Mannan Binding Lectin magnetic Beads, conjugated with Anti-Mannan Binding Lectin antibody, are used for immuneprecipitation (IP) of Mannan Binding Lectin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Mannan Binding Lectin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Mannan Binding Lectin proteins are dissociated from the magnetic beads using an elution buffer. Anti-Mannan Binding Lectin Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the C-terminus of the human MBL2
Species Reactivity
human MBL2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the C-terminus of the human MBL2, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Mannan Binding Lectin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-COLEC1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HSMBPCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MBLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MBL2DALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MBPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MBP-CALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MBP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MBPDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Mannan Binding Lectin Background Information
MBL (mannose-binding lectin) is primarily a liver-derived collagen-like serum protein, which binds sugar structures on micro-organisms and on dying host cells and is one of the four known mediators that initiate activation of the complement system via the lectin pathway. MBL and the ficolins (Ficolin-1, Ficolin-2 and Ficolin-3) are soluble collagen-like proteins that are involved in innate immune defence. They bind sugar structures or acetylated compounds present on microorganisms and on dying host cells and they initiate activation of the lectin complement pathway in varying degrees. MBL2 encodes the mannose-binding lectin, which is a key player in the innate immune system and has recently been found to play a role in development of type 1 diabetes and gestational diabetes mellitus. Common variant alleles situated both in promoter and structural regions of the MBL2 gene influence the stability and the serum concentration of the protein. Several polymorphisms in the promoter and structural regions of MBL2 adversely affect the plasma concentration and oligomeric state of MBL. The possession of mutant alleles has been linked to disease outcome for a variety of bacterial and viral infections. Mutant MBL2 haplotypes have been linked to disease progression and response to therapy in HCV infection.
Full Name
mannose-binding lectin (protein C) 2, soluble
References
Garred P, et al. (2006) Mannose-binding lectin and its genetic variants. Genes Immun. 7(2): 85-94. Brown KS, et al. (2007) Mannan binding lectin and viral hepatitis. Immunol Lett. 108(1): 34-44. Garred P. (2008) Mannose-binding lectin genetics: from A to Z. Biochem Soc Trans. 36(Pt 6): 1461-6. Garred P, et al. (2009) MBL2, FCN1, FCN2 and FCN3-The genes behind the initiation of the lectin pathway of complement. Mol Immunol. 46(14): 2737-44. Muller YL, et al. (2010) Functional Variants in MBL2 Are Associated With Type 2 Diabetes and Pre-Diabetes Traits in Pima Indians and the Old Order Amish. Diabetes. 59(8): 2080-5.
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