Anti-Smad3 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Smad3 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Smad3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Smad3 Magnetic Beads-IP Kit Product Description
The Anti-Smad3 magnetic Beads, conjugated with Anti-Smad3 antibody, are used for immuneprecipitation (IP) of Smad3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Smad3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Smad3 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Smad3 Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the center region of the human SMAD3
Species Reactivity
human SMAD3
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the human SMAD3, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Smad3 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-HSPC193ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HsT17436ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-JV15-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LDS1CALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LDS3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MADH3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Smad3 Background Information
SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD3 is involved in cell signalling. It modulates signals of activin and TGFβ's. Binding of SMAD3 with SMAD4 enables its transmigration into the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD3 is a receptor-regulated SMAD (R-SMAD). In mice, mutation of SMAD3 has been linked to colorectal adenocarcinoma, increased systemic inflammation, and accelerated wound healing. Increased SMAD3 activity has been implicated in the pathogenesis of scleroderma. Smad3 is also a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes.
Full Name
SMAD family member 3
References
Tan. et al., 2011, Diabetes. 60 (2): 464-76. Yang X. et al., 1999, Nat Cell Biol. 1 (5): 260-6. Zhu Y. et al., 1998, Cell. 94 (6): 703-14. Feng X. et al., 1996, Nature. 383 (6596): 168-72.
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