Anti-2B4 / CD244 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-2B4 / CD244 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-2B4 / CD244 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-2B4 / CD244 Magnetic Beads-IP Kit Product Description
The Anti-2B4 / CD244 magnetic Beads, conjugated with Anti-2B4 / CD244 antibody, are used for immuneprecipitation (IP) of 2B4 / CD244 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing 2B4 / CD244 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound 2B4 / CD244 proteins are dissociated from the magnetic beads using an elution buffer. Anti-2B4 / CD244 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human 2B4 / CD244 protein (Catalog#10042-H08H)
Species Reactivity
Human 2B4 / CD244
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human 2B4 / CD244 (rh 2B4 / CD244; Catalog#10042-H08H; NP_057466.1; Met1-Arg221).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-2B4 / CD244 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-2B4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NAILALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NKR2B4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NmrkALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SLAMF4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
2B4 / CD244 Background Information
The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.
Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy Full Name
CD244 molecule, natural killer cell receptor 2B4
References
Nakajima H, et al. (2000) 2B4: an NK cell activating receptor with unique specificity and signal transduction mechanism. Hum Immunol. 61(1): 39-43. Chuang SS, et al. (2001) 2B4 (CD244)-mediated activation of cytotoxicity and IFN-gamma release in human NK cells involves distinct pathways. J Immunol. 167(11): 6210-6. McNerney ME, et al. (2005) 2B4 (CD244) is a non-MHC binding receptor with multiple functions on natural killer cells and CD8+ T cells. Mol Immunol. 42(4): 489-94. Mathew SO, et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur J Immunol. 39(6): 1632-41.
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