Anti-ADAM17 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ADAM17 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ADAM17 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ADAM17 Magnetic Beads-IP Kit Product Description
The Anti-ADAM17 magnetic Beads, conjugated with Anti-ADAM17 antibody, are used for immuneprecipitation (IP) of ADAM17 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ADAM17 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ADAM17 proteins are dissociated from the magnetic beads using an elution buffer. Anti-ADAM17 Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the C-terminus of the human CD156b
Species Reactivity
Human ADAM17 / CD156b
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human CD156b, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ADAM17 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ADAM18ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD156BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CSVPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NISBDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NISBD1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TACEALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ADAM17 Background Information
ADAM17 is a member of the ADAM protein family of disintegrins and metalloproteases. ADAM17 is ubiquitously expressed in the human colon, with increased activity in the colonic mucosa of patients with ulcerative colitis, a main form of inflammatory bowel disease. The expression of ADAM17 may be inhibited by ethanol. It is involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. ADAM17 also plays a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes. ADAM17 may play a prominent role in the Notch signaling pathway, during the proteolytic release of the Notch intracellular domain (from the Notch1 receptor) that occurs following ligand binding.
Full Name
ADAM metallopeptidase domain 17
References
Poghosyan. et al., 2002, J Biol Chem. 277 (7): 4999-5007. Li Y. et al., 2006, Blood. 108 (7): 2275-9. Peiretti. et al., 2003, J Cell Sci. 116 (10): 1949-57.
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