Anti-Glypican 3 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Glypican 3 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Glypican 3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Glypican 3 Magnetic Beads-IP Kit Product Description
The Anti-Glypican 3 magnetic Beads, conjugated with Anti-Glypican 3 antibody, are used for immuneprecipitation (IP) of Glypican 3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Glypican 3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Glypican 3 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Glypican 3 Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human Glypican 3 / GPC3 / OCI-5
Species Reactivity
Human Glypican 3 / GPC3 / OCI-
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Glypican 3 / GPC3 / OCI-5, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Glypican 3 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-DGSXALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Glypican 3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GTR2-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MXR7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-OCI-5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SDYSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SGBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SGBSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SGBS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Glypican 3 Background Information
Glypican-3, also known as Intestinal protein OCI-5, GPC3, and OCI5, is a member of the glypican family. It belongs to the glypican family and is highly expressed in lung, liver, and kidney. It is a heparan sulfate proteoglycan, which is overexpressed in various neoplasms such as hepatocellular carcinoma, malignant melanoma, and testicular yolk sac tumor, and plays an important role in cell growth and differentiation. GPC3 function is tissue dependent. In some tissues, GPC3 acts as a tumor suppressor gene, whereas in others, it acts as an oncofetal protein. Studies have shown that GPC3 is a reliable marker for hepatocellular carcinoma. The sensitivity and specificity exceeds both alpha-fetoprotein and hepatocyte-paraffin1. GPC3 immunohistochemistry can aid in the differentiation of testicular germ cell tumors, being expressed in all yolk sac tumors but not in seminomas. GPC3 expression has also been identified in some squamous cell carcinomas of the lung and clear cell carcinomas of the ovary. The role of GPC3 in melanomas is still controversial. Thus, Glypican-3 is currently regarded as a tumor marker and potential target for immunotherapy.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy References
Kandil DH, et al. (2009) Glypican-3: a novel diagnostic marker for hepatocellular carcinoma and more. Adv Anat Pathol. 16(2): 125-9. Maeda D, et al. (2009) Glypican-3 expression in clear cell adenocarcinoma of the ovary. Mod Pathol. 22(6): 824-32.
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