Anti-CEBP alpha Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CEBP alpha Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CEBP alpha magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CEBP alpha Magnetic Beads-IP Kit Product Description
The Anti-CEBP alpha magnetic Beads, conjugated with Anti-CEBP alpha antibody, are used for immuneprecipitation (IP) of CEBP alpha proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CEBP alpha proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CEBP alpha proteins are dissociated from the magnetic beads using an elution buffer. Anti-CEBP alpha Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the C-terminus of the human CEBPA .
Species Reactivity
Huamn CEBPA
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the C-terminus of the human CEBPA . Huamn CEBPA specific IgG was purified by Huamn CEBPA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CEBP alpha Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-C/EBP-alphaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CEBPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CEBP alpha Background Information
The CCAAT/enhancer-binding protein-alpha (CEBPA) is lineage-specific transcription factor in the hematopoietic system. The presence of biallelic CEBPA mutations is a favourable prognostic feature in acute myeloid leukaemia (AML). CEBPA mutations are currently identified through conventional capillary sequencing (CCS). Mutations in CCAAT/enhancer binding protein alpha (CEBPA) occur in 5-1% of cases of acute myeloid leukemia. CCAAT/enhancer binding protein alpha gene (CEBPA) is an important transcription factor in maintenance of differentiation of granulocyte series of hematopoietic system. It plays a key role in regulating cell proliferation and differentiation.
Full Name
CCAAT/enhancer binding protein alpha
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