Anti-BCRP / ABCG2 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-BCRP / ABCG2 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-BCRP / ABCG2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-BCRP / ABCG2 Magnetic Beads-IP Kit Product Description
The Anti-BCRP / ABCG2 magnetic Beads, conjugated with Anti-BCRP / ABCG2 antibody, are used for immuneprecipitation (IP) of BCRP / ABCG2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing BCRP / ABCG2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound BCRP / ABCG2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-BCRP / ABCG2 Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human ABCG2
Species Reactivity
Human ABCG2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ABCG2, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-BCRP / ABCG2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ABC15ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ABCPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-BCRPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-BCRP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-BMDPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD338ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CDw338ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EST157481ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GOUT1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MRXALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MXRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MXR1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UAQTL1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
BCRP / ABCG2 Background Information
ATP-binding cassette subfamily G member 2 (ABCG2), a member of the ABC transporter superfamily proteins. the expression of ABCG2 is related to tumorigenic cancer stem cells (CSC) in several cancers, mediates multidrug resistance (MDR) by transporting substrate anticancer drugs out of cancer cells and decreasing their intracellular accumulation. ABCB1 and ABCG2 are efflux transporters which have a major impact on the pharmacological behavior of numerous drugs. They are expressed in the intestine, liver, kidney, BBB and placenta. It has become evident that ABCB1 and ABCG2 modify the pharmaco/toxicokinetics in the placenta and fetus and may consequently affect the outcome of pregnancy.
Full Name
ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group)