Anti-Noggin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Noggin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Noggin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Noggin Magnetic Beads-IP Kit Product Description
The Anti-Noggin magnetic Beads, conjugated with Anti-Noggin antibody, are used for immuneprecipitation (IP) of Noggin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Noggin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Noggin proteins are dissociated from the magnetic beads using an elution buffer. Anti-Noggin Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the N-terminus of the human NOG
Species Reactivity
Human NOG
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the human NOG, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Noggin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-NogginALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SYM1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SYNS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Noggin Background Information
Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities, with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs, Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7, and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes, including embryonic dorsal-ventral axis, induction of neural tissue, formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion.
References
Zimmerman LB, et al. (1996) The Spemann organizer signal noggin binds and inactivates bone morphogenetic protein 4. Cell. 86(4): 599-606. Chandramore K, et al. (2010) Cloning of noggin gene from hydra and analysis of its functional conservation using Xenopus laevis embryos. Evol Dev. 12(3): 267-74.
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