Anti-Cytokeratin 5 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-Cytokeratin 5 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Cytokeratin 5 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Cytokeratin 5 Magnetic Beads-IP Kit Product Description
The Anti-Cytokeratin 5 magnetic Beads, conjugated with Anti-Cytokeratin 5 antibody, are used for immuneprecipitation (IP) of Cytokeratin 5 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Cytokeratin 5 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Cytokeratin 5 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Cytokeratin 5 Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the center region of the Human Cytokeratin 5 / KRT5
Species Reactivity
Human Cytokeratin 5 / KRT5
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Cytokeratin 5 / KRT5, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Cytokeratin 5 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CK5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DDDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DDD1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EBS2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-K5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-KRT5AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Cytokeratin 5 Background Information
Keratin 5 is the major type II keratin of the basal cells of epidermis and of other stratified epithelia. With its type I partner, keratin 14, it constitutes a major fraction of the cytoskeleton of the basal cells. Epidermolysis bullosa simplex with mottled pigmentation (EBS-MP) is a rare dermatologic disorder of autosomal dominant inheritance with intraepidermal blistering after minor trauma, reticular hyperpigmentation unrelated to the blistering, nail dystrophy, and mild palmoplantar keratosis. Keratin 5 and keratin 14 are known to be essential for the basal keratinocyte cytoskeleton and are defective in several forms of epidermolysis bullosa simplex. Recently, a 71C-->T transition in the keratin 5 gene (KRT5) causing a P24L substitution was identified in some patients with EBS-MP.
Full Name
keratin 5, type II