Anti-Cytokeratin 7 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Cytokeratin 7 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Cytokeratin 7 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Cytokeratin 7 Magnetic Beads-IP Kit Product Description
The Anti-Cytokeratin 7 magnetic Beads, conjugated with Anti-Cytokeratin 7 antibody, are used for immuneprecipitation (IP) of Cytokeratin 7 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Cytokeratin 7 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Cytokeratin 7 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Cytokeratin 7 Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the center region of the human KRT7
Species Reactivity
Human KRT7
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the human KRT7 , and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Cytokeratin 7 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CK7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-K2C7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-K7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SCLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Cytokeratin 7 Background Information
One AS, designated Homo sapiens keratin 7 (KRT7-AS), was selected due to its marked upregulation and concordant expression with its cognate sense counterpart, KRT7, in GC tissues and cell lines. KRT7-AS formed an RNA-RNA hybrid with KRT7 and controlled KRT7 expression at both the mRNA and the post-transcriptional levels. Moreover, forced overexpression of the KRT7-overlapping region (OL) of KRT7-AS (but not its non-KRT7-OL portions) increased keratin 7 protein levels in cells. Finally, forced overexpression of full-length KRT7-AS or OL KRT7-AS (but not its non-KRT7-OL regions) promoted GC cell proliferation and migration.
Full Name
keratin 7, type II
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