Anti-Cytokeratin 19 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Cytokeratin 19 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Cytokeratin 19 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Cytokeratin 19 Magnetic Beads-IP Kit Product Description
The Anti-Cytokeratin 19 magnetic Beads, conjugated with Anti-Cytokeratin 19 antibody, are used for immuneprecipitation (IP) of Cytokeratin 19 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Cytokeratin 19 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Cytokeratin 19 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Cytokeratin 19 Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the C-terminus of the human Cytokeratin 19.
Species Reactivity
Human Cytokeratin 19
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the C-terminus of the human Cytokeratin 19. Human Cytokeratin 19 specific IgG was purified by Human Cytokeratin 19 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Cytokeratin 19 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CK19ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-K19ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-K1CSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Cytokeratin 19 Background Information
Cytokeratin 19 (KRT19) is a cytoplasmic intermediate filament protein, which is responsible for structural rigidity and multipurpose scaffolds. In several cancers, KRT19 is overexpressed and may play a crucial role in tumorigenic transformation.The KRT19 gene encodes cytokeratin 19, an element of the cytoskeleton whose expression is frequently altered in renal cell carcinoma (RCC).KRT19 was identified as one of the key biomarkers for distinguishing cholangiocarcinoma (CCA) and hepatocellular carcinoma.Expression of KRT19 was specifically high in breast cancers and significantly correlated with their invasiveness. Moreover, knockdown of KRT19 led to increased proliferation, migration, invasion, drug resistance, and sphere formation in breast cancer cells via an upregulated NOTCH signaling pathway.
Full Name
keratin 19, type I
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