Anti-CXCL1 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-CXCL1 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CXCL1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CXCL1 Magnetic Beads-IP Kit Product Description
The Anti-CXCL1 magnetic Beads, conjugated with Anti-CXCL1 antibody, are used for immuneprecipitation (IP) of CXCL1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CXCL1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CXCL1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CXCL1 Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the center region of the Human CXCL1
Species Reactivity
Human CXCL1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human CXCL1, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CXCL1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CA21HALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CAH1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CPS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CYP21ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CYP21BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FSPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GRO1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GROaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MGSAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MGSA-aALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NAP-3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-P450c21BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SCYB1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CXCL1 Background Information
The Chemokine (C-X-C motif) Ligand 1, CXCL1, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a). CXCL1 already known to be important in osteoarthritis (OA), as a novel target gene of transcription factor AP-2? in chondrocytes and support the important role of AP-2? in cartilage. CXCL1 is a potent neutrophil chemoattractant with recognized roles in angiogenesis and inflammation. CXCL1 is a novel immediate PTH/PTHrP-responsive gene. CXCL1 may act as a chemoattractant for osteoclast precursors. CXCL1 may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. CXCL1 functions through CXCR2 to transactivate the EGFR by proteolytic cleavage of HB-EGF, leading to activation of MAPK signalling and increased proliferation of epithelial ovarian cancer (EOC) cells. It might limit tumor growth by reinforcing senescence early in tumorigenesis. Thus, CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.
Full Name
chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
References
Wang JG, et al. (2008) The chemokine CXCL1/growth related oncogene increases sodium currents and neuronal excitability in small diameter sensory neurons. Mol Pain. 4: 38. Acosta JC, et al. (2009) A role for CXCR2 in senescence, but what about in cancer? Cancer Res. 69(6): 2167-70. Onan D, et al. (2009) The chemokine Cxcl1 is a novel target gene of parathyroid hormone (PTH)/PTH-related protein in committed osteoblasts. Endocrinology. 150(5): 2244-53. Ritzman AM, et al. (2010) The chemokine receptor CXCR2 ligand KC (CXCL1) mediates neutrophil recruitment and is critical for development of experimental Lyme arthritis and carditis. Infect Immun. 78(11): 4593-600. Bolitho C, et al. (2010) The chemokine CXCL1 induces proliferation in epithelial ovarian cancer cells by transactivation of the epidermal growth factor receptor. Endocr Relat Cancer. 17(4): 929-40. Wenke AK, et al. (2011) The transcription factor AP-2? regulates CXCL1 during cartilage development and in osteoarthritis. Osteoarthritis Cartilage. 19(2): 206-12.