Anti-Bcl-2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Bcl-2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Bcl-2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Bcl-2 Magnetic Beads-IP Kit Product Description
The Anti-Bcl-2 magnetic Beads, conjugated with Anti-Bcl-2 antibody, are used for immuneprecipitation (IP) of Bcl-2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Bcl-2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Bcl-2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Bcl-2 Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Bcl-2.
Species Reactivity
Human Bcl-2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the N-terminus of the Human Bcl-2. Human Bcl-2 specific IgG was purified by Human Bcl-2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Bcl-2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-Bcl-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PPP1R50ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Bcl-2 Background Information
BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy Full Name
B-cell CLL/lymphoma 2
References
Tsujimoto Y, et al. (1984) Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation. Science. 226(4678):1097-99. Cleary ML, et al. (1986) Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell. 47(1):19-28. Otake Y, et al. (2007) Overexpression of nucleolin in chronic lymphocytic leukemia cells induces stabilization of Bcl-2 / Bcl-2 mRNA. Blood. 109(7):3069-75.
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