Anti-SOX2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SOX2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SOX2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SOX2 Magnetic Beads-IP Kit Product Description
The Anti-SOX2 magnetic Beads, conjugated with Anti-SOX2 antibody, are used for immuneprecipitation (IP) of SOX2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SOX2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SOX2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SOX2 Magnetic Beads-IP Kit Antibody Information
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Sox2.
Species Reactivity
Human Sox2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Sox2, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SOX2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ANOP3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MCOPS3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SOX2 Background Information
SOX2, also known as ANOP3, is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. SOX2 is critical for early embryogenesis and for embryonic stem cell pluripotency. It keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Defects in SOX2 can cause microphthalmia, syndromic, 3. A disease characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Full Name
SRY (sex determining region Y)-box 2
References
Lu Y, et al., 2010, PLoS ONE. 5(6): e11022. Gontan C, et al., 2008, Dev. Biol. 317 (1): 296-309. Shi W, et al., 2006, J. Biol. Chem. 281 (33): 23319-25.
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