Anti-RAD18 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-RAD18 Antibody
Validated applications
IHC-P,ICC/IF,IF
Species reactivity
Reacts with: Human
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human RAD18
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human RAD18, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-RAD18 Antibody (Rabbit Polyclonal antibody) Validated Applications
| Application | Dilution | Notes |
| IHC-P | 1:500-1:2000 | |
| ICC-IF | 1:300-1:10000 | |
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-RAD18 Antibody (Rabbit Polyclonal antibody) Images
Immunofluorescence staining of RAD18 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human RAD18 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.
Immunochemical staining of human RAD18 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Anti-RAD18 Antibody Alternative Names
Anti-RNF73 Antibody
RAD18 Background Information
RAD18, a RING-type ubiquitin ligase (E3) that plays an essential role in post-replication repair, possesses distinct domains named RING, UBZ, SAP and the RAD6-binding domain (R6BD) and forms a dimer. RAD18, a DNA damage-activated E3 ubiquitin ligase, is known to play a critical role in DNA damage repair in cancer cells.The ubiquitin ligase RAD18 is involved in post replication repair pathways via its recruitment to stalled replication forks, and its role in the ubiquitylation of proliferating cell nuclear antigen (PCNA).RAD18 is an ubiquitin ligase involved in replicative damage bypass and DNA double-strand break (DSB) repair processes.The RAD18 gene, located on the human chromosome 3p24-p25, plays a crucial role in post-replication repair (PRR) in various organisms from yeast to humans. In the human RAD18 gene, one coding single nucleotide polymorphism (SNP) at codon 32, encoding either arginine (Arg, CGA) or glutamine (Gln, CAA), was reported.
Full Name
RAD18, E3 ubiquitin protein ligase
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