SUMO1 cDNA ORF Clone, Human, N-OFPSpark® tag 基本信息
基因
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human small ubiquitin-like modifier 1 with N terminal OFPSpark / RFP tag.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI(6kb+0.99kb)
标签序列
OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
SUMO1 cDNA ORF Clone, Human, N-OFPSpark® tag Validated Images
SUMO1 cDNA ORF Clone, Human, N-OFPSpark® tag Alternative Names
DAP1 cDNA ORF Clone, Human;GMP1 cDNA ORF Clone, Human;OFC10 cDNA ORF Clone, Human;PIC1 cDNA ORF Clone, Human;SENP2 cDNA ORF Clone, Human;SMT3 cDNA ORF Clone, Human;SMT3C cDNA ORF Clone, Human;SMT3H3 cDNA ORF Clone, Human;UBL1 cDNA ORF Clone, Human
SUMO1 Background Information
Small ubiquitin-like modifier protein (SUMO) modification is a highly dynamic process, catalyzed by SUMO-specific activating (E1), conjugating (E2) and ligating (E3) enzymes, and reversed by a family of SUMO-specific proteases (SENPs). Small ubiquitin-like modifier 1 (SUMO1) is a member of the superfamily of ubiquitin-like proteins. Despite its structural similarity with ubiquitin, SUMO1 does not seem to play any role in protein degradation. SUMO1 plays an important role in modulation of NOX activity required for ROS generation. SUMO1 haploinsufficiency results in cleft lip and palate in animal models. SUMO1 gene variation in human non-syndromic cleft lip with or without cleft palate (NSCLP) development. SUMO-1 may be useful as a novel target for therapy in oral squamous cell carcinoma (SCC) as well as a clinical indicator for tumor recurrence together with Mdm2.
Full Name
small ubiquitin-like modifier 1
References
Kim HJ, et al. (2011) SUMO1 attenuates stress-induced ROS generation by inhibiting NADPH oxidase 2. Biochem Biophys Res Commun. 410(3): 555-62. Zuo Y, et al. (2009) Small ubiquitin-like modifier protein-specific protease 1 and prostate cancer. Asian J Androl. 11(1): 36-8. Song T, et al. (2008) SUMO1 polymorphisms are associated with non-syndromic cleft lip with or without cleft palate. Biochem Biophys Res Commun. 377(4): 1265-8. Katayama A, et al. (2007) Overexpression of small ubiquitin-related modifier-1 and sumoylated Mdm2 in oral squamous cell carcinoma: possible involvement in tumor proliferation and prognosis. Int J Oncol. 31(3): 517-24.
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