pCMV/hygro-Positive Control Vector (C-terminal Fc-HA tag) 1Unit

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pCMV/hygro-Positive Control Vector (C-terminal Fc-HA tag) 1Unit

市场价：: 960.00

促销价：: ¥864.00

货号：CV007

品牌：义翘神州

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计5-7工作日到货)

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

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Positive control for the pCMV / hygro-HA.
Designed for mammalian expression, stable or transient.
Hygromycin resistance gene for selection of stable cell lines.

pCMV / hygro-Positive Control Vector (C-terminal Fc-HA tag) Physical Map

Description

Vector Name	pCMV / hygro-Positive Control Vector (C-terminal Fc-HA tag)
Vector Size	6335bp
Vector Type	Mammalian Expression Vector
Expression Method	Constitutive, Stable / Transient
Promoter	CMV
Antibiotic Resistance	Ampicillin
Selection In Mammalian Cells	Hygromycin
Protein Tag	TATCCTTACGACGTGCCTGACTAC
Sequencing Primer	Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV / hygro-Positive Control Vector (C-terminal Fc-HA tag) Sequence and Quality Control

1        ATGGGCTGGT CCTGCATCAT CCTGTTCCTC GTGGCGACCG CGACCGGGGT CCACAGC¹GAG61       CCCAAATCTT CTGACAAAAC TCACACATGC CCACCGTGCC CAGCACCTGA ACTCCTGGGG121      GGACCGTCAG TCTTCCTCTT CCCCCCAAAA CCCAAGGACA CCCTCATGAT CTCCCGGACC181      CCTGAGGTCA CGTGCGTGGT GGTGGACGTG AGCCACGAAG ACCCCGAGGT CAAGTTCAAC241      TGGTACGTGG ACGGCGTGGA GGTGCATAAT GCCAAGACAA AGCCGCGGGA GGAGCAGTAC301      AACAGCACGT ACCGTGTGGT CAGCGTCCTC ACCGTCCTGC ACCAGGACTG GCTGAATGGC361      AAGGAGTACA AGTGCAAGGT CTCCAACAAA GCCCTCCCAG CCCCCATCGA GAAAACCATC421      TCCAAAGCCA AAGGGCAGCC CCGAGAACCA CAGGTGTACA CCCTGCCCCC ATCCCGGGAT481      GAGCTGACCA AGAACCAGGT CAGCCTGACC TGCCTGGTCA AAGGCTTCTA TCCCAGCGAC541      ATCGCCGTGG AGTGGGAGAG CAATGGGCAG CCGGAGAACA ACTACAAGAC CACGCCTCCC601      GTGCTGGACT CCGACGGCTC CTTCTTCCTC TACAGCAAGC TCACCGTGGA CAAGAGCAGG661      TGGCAGCAGG GGAACGTCTT CTCATGCTCC GTGATGCATG AGGCTCTGCA CAACCACTAC721      ACGCAGAAGA GCCTCTCCCT GTCTCCGGGT AAAGCT²TATC CTTACGACGT GCCTGACTAC781      GCC³TAA

1. Signal peptide
2. GCT was the nucleotide residue from the restriction site during plasmid construction, which has no influence on protein expression.
3. HA Tag

Detect Positive Control Vector Expression by Western Blot

Protocol :
The 6 µg of plasmid was transfected into 20 ml of HEK293H suspension cells with Sinofection reagent (Cat# STF01). Experssion cells were cultured for 4d at 37°C (5% CO ₂). The 2×10 ⁷ of cells were lysed in 1 ml of ice-cold modified RIPA Lysis Buffer with protease inhibitors cocktail (Sigma) by homogenization. The protein concentration of cell lysate was measured by BCA kit, and 1~5 µg of lysate were detected by western blotting using specific anti-tag antibody.