Human SLITRK1 HEK293 Overexpression Lysate 产品信息
Product Description
This Human SLITRK1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SLITRK1 protein (Cat: 10340-H08H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Sequence Information
A DNA sequence encoding the extracellular domain (Met 1-Ser 616) of human SLITRK1 (NP_443142.1) was expressed with a C-terminal polyhistidine tag.
Molecule Mass
The recombinant human SLITRK1 consists of 610 amino acids and predicts a molecular mass of kDa. As a result of glycosylation, the apparent molecular mass of rhSLITRK1 is approximately 105-115 kDa in SDS-PAGE under reducing conditions.
Human SLITRK1 HEK293 Overexpression Lysate Usage Guide
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
Human SLITRK1 HEK293 Overexpression Lysate Alternative Names
Human FLJ54428 Overexpression Lysate;Human KIAA0918 Overexpression Lysate;Human KIAA1910 Overexpression Lysate;Human LRRC12 Overexpression Lysate;Human RP11-395N17.1 Overexpression Lysate;Human SLITRK1 Overexpression Lysate;Human TTM Overexpression Lysate
SLITRK1 Background Information
SLITRK1 (Slit and Trk-like family member 1) is a integral membrane protein belonging to the SLITRK family consists of at least 6 members (SLITRK1-6). They are named and characterized by the presence of two leucine-rich repeats (LRRs) in the extracellular domain similar to those found in a secreted axonal growth-controlling protein, Slit, as well as a C-terminal domain with homology to Trk neurotrophin tyrosine kinase receptors. Expression of SLITRKs are highly restricted to neural tissues, and are identified as the neuronal components modulating the neurite outgrowth. More specifically, SLITRK1 expression is found in the mature neurons of the cerebrum, thalamus and hippocampus, and induces unipolar neurites in cultured neuronal cells. Human SLITRK1 is a 696 amino acid precursor protein, and one truncating frameshift mutation (448 aa) has been linked to Tourette's syndrome, a genetically influenced developmental neuropsychiatric disorder characterized by chronic vocal and motor tics. In addition, all SLITRK genes are differentially expressed in brain tumors, such as astrocytoma, oligodendroglioma, glioblastoma, and are suggested to be useful molecular indicators of brain tumor properties.
Full Name
SLIT and NTRK like family member 1
References
1. Aruga, J. and Mikoshiba, K. 2003, Mol. Cell. Neurosci. 24: 117-129.
2. Aruga, J. et al., 2003, Gene. 315: 87-94.
3. Abelson, J.F. et al., 2005, Science. 310: 317-320.
4. Grados, M.A. and Walkup. J.T. 2006, Trends. Genet. 22: 291-293.
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