eIF5A qPCR Primer Pairs, Mouse 基本信息
Target Details
Product Details
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
应用:
SYBR® Green-based quantitative real-time PCR (qPCR).
质控:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
运输:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
储存:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***
Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
eIF5A qPCR Primer Pairs, Mouse Alternative Names
AA410058 qPCR Primer Pairs, Mouse;D19Wsu54e qPCR Primer Pairs, Mouse;eIF-4D qPCR Primer Pairs, Mouse;eIF-5A qPCR Primer Pairs, Mouse;eIF-5A-1 qPCR Primer Pairs, Mouse;eIF-5A1 qPCR Primer Pairs, Mouse;Eif4d qPCR Primer Pairs, Mouse;Eif5a1 qPCR Primer Pairs, Mouse
eIF5A Background Information
EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2?GTP?tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 4S ribosome subunit.
Full Name
eukaryotic translation initiation factor 5A
References
Jenkins ZA. et al., 2001, Genomics. 71 (1): 101-9. Guan XY. et al., 2001, Cancer Res. 61 (9): 3806-9. Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903. Clement PM. et al., 2003, Eur J Biochem. 270 (21): 4254-63.