Peroxiredoxin 5 cDNA ORF Clone, Human, C-OFPSpark® tag 基本信息
基因
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human peroxiredoxin 5 with C terminal OFPSpark / RFP tag.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI (6kb + 1.34kb)
标签序列
OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
Peroxiredoxin 5 cDNA ORF Clone, Human, C-OFPSpark® tag Validated Images
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.
Peroxiredoxin 5 cDNA ORF Clone, Human, C-OFPSpark® tag Alternative Names
ACR1 cDNA ORF Clone, Human;AOEB166 cDNA ORF Clone, Human;B166 cDNA ORF Clone, Human;HEL-S-55 cDNA ORF Clone, Human;PLP cDNA ORF Clone, Human;PMP20 cDNA ORF Clone, Human;PRDX6 cDNA ORF Clone, Human;prx-V cDNA ORF Clone, Human;PRXV cDNA ORF Clone, Human;SBBI10 cDNA ORF Clone, Human
Peroxiredoxin 5 Background Information
Peroxiredoxin-5, also known as Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, Peroxisomal antioxidant enzyme, Thioredoxin peroxidase PMP20, Thioredoxin reductase, PRDX5 and ACR1, is cytoplasm protein which belongs to the?peroxiredoxin 2 family. Peroxiredoxin-5 / PRDX5 reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-5 / PRDX5 is involved in intracellular redox signaling. The Peroxiredoxins / Prx are a family of 25 kDa peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins / Prx family is divided into six groups ( PRDX1,PRDX2, PRDX3, PRDX4, PRDX5, PRDX6 ) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins / Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins / Prx has a proliferative effect and may be related to cancer development or progression.
Full Name
peroxiredoxin 5
References
Seo M.S., et al., 2000, J. Biol. Chem. 275: 20346-54. Declercq J.-P., et al., 2001, J. Mol. Biol. 311:751-9. Noh,D.Y. et al., 2001, Anticancer Res. 21 (3B): 2085-90. Schremmer,B. et al., 2007, Subcell Biochem. 44 :317-44.