描述
The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 2 tubes of RNase inhibitor (100 µL each).
The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.
Features of this kit include:
• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits
Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA. 规格
Reverse Transcriptase: | MultiScribe™ |
Downstream Application: | Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR) |
Reaction Format: | Separate Components |
Sample Type (General): | RNA |
Final Product: | cDNA (First-Strand) |
Final Product Size(s): | 7 kb or less |
Optimal Reaction Temperature: | 37° C |
Product Size: | 1,000 reactions |
内容及储存
Contents:
• 10X RT Buffer (2 × 1 mL)
• 10X RT Random Primers (2 × 1 mL)
• 25X dNTP Mix (100 mM, 1 mL)
• MultiScribe® Reverse Transcriptase (1 mL at 50 U/µL)
• RNase Inhibitor (10 × 0.1 mL at 20 U/µL)
Store between -15°C and -25°C.