Description

The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but may be used with any fluorometer fluorescence plate reader. The Qubit® ssDNA Kit is ideal for quantitating single-stranded DNA oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA RNA but it will not detect contaminating protein nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg/µL to 200 ng/µL providing an assay range of 1–200 ng. The kit provides concentrated assay reagent, dilution buffer, pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl 20 µl is acceptable), read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, protein are well tolerated in the assay.

Which product to choose for ssDNA quantitation?
• For 1–20 samples: use this Qubit ssDNA Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT OliGreen ssDNA Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

 

Specifications

Sample Type (General):	ssDNA
For Use With (Equipment):	Qubit® Fluorometer
Quantitation Range:	1-200 ng
Number of Reactions:	100 reactions
Product Line:	Quant-iT™
Product Size:	1 kit
Shipping Condition:	Room Temperature

Contents & storage