描述

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension adherent mammalian cells, including keratinocytes, primary rat astrocytes, human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.

Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 other DHFR negative cells. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing Quality System
Gibco® MEM α is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® MEM α product made in our Scotland facility (11900-016). This facility is registered with the FDA as a medical device manufacturer is certified to the ISO 13485 standard.

MEM α contains no proteins, lipids, growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, filtration at the time of preparation (see protocol for details).