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Format
96-well strip plate
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Assay Length
4.5 hours
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Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL)
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Sensitivity
0.041 ng/mL
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Assay Range
0.0 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Specificity
Measures Testosterone levels in various samples
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Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
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Interference
Interference observed with 1 or more available related molecules.
Control Available
QC165, Parameter Immunoassay Control Set 891 for Testosterone -
Product Summary
The Parameter Testosterone kit is a 4.5 hour competitive enzyme immunoassay designed to measure Testosterone in cell culture supernates, serum, and plasma.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1.25 | 3.5 | 5.8 | 1.26 | 3.67 | 6.03 |
| Standard Deviation | 0.05 | 0.11 | 0.17 | 0.07 | 0.23 | 0.41 |
| CV% | 4 | 3.1 | 2.9 | 5.6 | 6.3 | 6.8 |
Recovery
The recovery of Testosterone spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 103 | 95-117 |
| EDTA Plasma (n=4) | 94 | 85-107 |
| Heparin Plasma (n=4) | 92 | 84-101 |
| Serum (n=4) | 93 | 86-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Testosterone were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: Testosterone
Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.
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Alternate Names
Testosterone;
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