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Assay Length
4.5 hours
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Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
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Sensitivity
10.9 pg/mL
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Assay Range
10.3 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Specificity
Measures LTB 4 levels in various samples
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Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
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Interference
No significant interference observed with available related molecules.
Control Available
QC116, Parameter Immunoassay Control Set 594 for LTB4 -
Product Summary
The Parameter LTB 4 Immunoassay is a 4.5 hour forward sequential competitive enzyme immunoassay designed to measure LTB4 in cell culture supernates, serum, and plasma.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 170 | 481 | 847 | 164 | 516 | 879 |
| Standard Deviation | 10 | 19 | 34 | 14.1 | 36.6 | 73.6 |
| CV% | 5.8 | 4 | 4 | 8.6 | 7.1 | 8.4 |
Recovery
The recovery of LTB4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 95 | 86-114 |
| EDTA Plasma (n=4) | 91 | 82-112 |
| Heparin Plasma (n=4) | 98 | 82-115 |
| Serum (n=4) | 98 | 82-118 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of LTB 4 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: Leukotriene B4/LTB4
Leukotriene B4 (LTB4) is a major product of arachidonic acid metabolism and is formed via the 5-lipoxygenase pathway. LTB4 stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation.
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Alternate Names
Leukotriene B4; LTB4;
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