Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 26 | 21 | 27 |
Mean | 33.3 | 56.4 | 397 | 36.5 | 62.7 | 405 |
Standard Deviation | 2.3 | 3 | 17.2 | 3.8 | 6.5 | 33.7 |
CV% | 6.9 | 5.3 | 4.3 | 10.4 | 10.4 | 8.3 |
The recovery of mouse IL-12/IL-23 p40 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Supernates (n=4) | 104 | 98-108 |
EDTA Plasma (n=4) | 99 | 81-112 |
Heparin Plasma (n=4) | 95 | 80-109 |
Serum (n=4) | 105 | 86-117 |
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
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