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Assay Length
3.5 hours
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Sample Type & Volume Required Per Well
Cell Culture Supernates (51 uL), Serum (51 uL), EDTA Plasma (51 uL), Heparin Plasma (51 uL), Urine (51 uL)
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Sensitivity
41.4 pg/mL
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Assay Range
39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Specificity
Measures PGE 2 levels in various samples
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Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
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Interference
No significant interference observed with available related molecules.
Control Available
QC142, Parameter Immunoassay Control Set 777 Human Prostaglandin E2 -
Product Summary
The Parameter PGE 2 Immunoassay is a 3.5 hour forward sequential competitive enzyme immunoassay designed to measure PGE2 in cell culture supernates, serum, plasma, and urine.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 234 | 895 | 1456 | 253 | 835 | 1331 |
| Standard Deviation | 21 | 45 | 89 | 33 | 82 | 120 |
| CV% | 9 | 5 | 6.1 | 12.9 | 9.9 | 9 |
Recovery
The recovery of PGE2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 97 | 84-106 |
| EDTA Plasma (n=4) | 93 | 82-108 |
| Heparin Plasma (n=4) | 94 | 81-111 |
| Serum (n=4) | 97 | 86-114 |
| Urine (n=4) | 99 | 81-111 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of PGE 2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: Prostaglandin E2/PGE2
Prostaglandin E2 (PGE2) is formed in a variety of cells from prostaglandin H2, which is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of human immunodeficiency virus replication. It elevates cAMP levels, stimulates bone resorption and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.
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