Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.
*Please allow additional time when placing orders through a distributor.
The following chart indicates the bead region, sensitivity, high standard value, and recommended samples and their starting dilution for each analyte.
Analyte | Sensitivity (MDD)pg/mL | Standard Curve Range (pg/mL) | Starting Sample Dilution | Magnetic Bead Region | |
---|---|---|---|---|---|
Cell Culture Supernates/ Lysates | Serum/ Plasma | ||||
Adiponectin | 7.55 | 18.6-4,520 | 1:2 | 1:4,000 | 34 |
Angiopoietin-2 | 30.8 | 638-155,080 | 1:2 | 1:2 | 67 |
BAFF/BLyS | 11.1 | 56.2-13,660 | 1:2 | 1:2 | 57 |
ß-NGF | 1.06 | 12.3-3,000 | 1:2 | 1:2 | 43 |
C1q R1/CD93 | 5.33 | 336-81,680 | 1:2 | 1:2 | 64 |
CCL2/JE/MCP-1 | 134 | 221-53,720 | 1:2 | 1:2 | 18 |
CCL3/MIP-1 alpha | 0.452 | 0.905-220 | 1:2 | 1:2 | 46 |
CCL4/MIP-1 beta | 77.4 | 169-41,160 | 1:2 | 1:2 | 51 |
CCL5/RANTES | 19.1 | 88.9-21,610 | 1:2 | 1:2 | 38 |
CCL7/MARC | 1.69 | 7.86-1,910 | 1:2 | 1:2 | 39 |
CCL8/MCP-2 | 0.283 | 8.15-1,980 | 1:2 | 1:200 | 38 |
CCL11/Eotaxin | 1.46 | 18.6-4,530 | 1:2 | 1:2 | 74 |
CCL12/MCP-5 | 0.613 | 8.23-2,000 | 1:2 | 1:2 | 42 |
CCL19/MIP-3 beta | 2.28 | 9.88-2,400 | 1:2 | 1:2 | 19 |
CCL20/MIP-3 alpha | 39.5 | 117-28,430 | 1:2 | 1:2 | 48 |
CCL21/6Ckine | 0.061 | 2.51-610 | 1:2 | 1:50 | 72 |
CCL22/MDC | 0.965 | 28.4-6,910 | 1:2 | 1:2 | 75 |
Chitinase 3-like 1/YKL-40 | 18.2 | 77.0-18,700 | 1:2 | 1:50 | 20 |
CRP | 13.3 | 67.5-16,400 | 1:2 | 1:200 | 63 |
CXCL1/KC | 32.9 | 28.5-6,920 | 1:2 | 1:2 | 13 |
CXCL2/MIP-2 | 1.97 | 4.4-1,080 | 1:2 | 1:2 | 20 |
CXCL10/IP-10/ CRG-2 | 6.85 | 48-11,660 | 1:2 | 1:2 | 37 |
CXCL12/SDF-1 alpha | 19.1 | 280-67,930 | 1:2 | 1:2 | 54 |
CXCL13/BLC/BCA-1 | 19.3 | 187-45,530 | 1:2 | 1:2 | 21 |
CXCL16 | 1.47 | 9.01-2,190 | 1:2 | 1:2 | 22 |
Cystatin C | 2.61 | 60.8-14,780 | 1:2 | 1:200 | 30 |
DPPIV/CD26 | 135 | 394-95,790 | 1:2 | 1:2 | 25 |
Dkk-1 | 31.8 | 365-88,720 | 1:2 | 1:2 | 77 |
EGF | 0.089 | 0.535-130 | 1:2 | 1:2 | 26 |
EGF R | 25.4 | 683-165,910 | 1:2 | 1:200 | 22 |
EMMPRIN | 10.5 | 136-32,990 | 1:2 | 1:2 | 19 |
Endoglin | 6.15 | 124-30,150 | 1:2 | 1:2 | 33 |
Factor D/Adipsin | 390 | 4,730-1,149,300 | 1:2 | 1:200 | 75 |
FABP4/A-FABP | 5.40 | 34.1-8,290 | 1:2 | 1:50 | 13 |
Fas Ligand | 2.34 | 32.7-7,940 | 1:2 | 1:2 | 27 |
FGF-basic | 41.3 | 48.1-11,700 | 1:2 | 1:2 | 65 |
FGF-21 | 0.914 | 24.0-5,820 | 1:2 | 1:2 | 61 |
G-CSF | 0.774 | 14.7-3,580 | 1:2 | 1:2 | 39 |
GDF-15 | 0.630 | 14.7-3,570 | 1:2 | 1:2 | 15 |
Glucagon | 58.5 | 136-32,940 | 1:2 | 1:2 | 34 |
GM-CSF | 1.64 | 11.6-2,830 | 1:2 | 1:2 | 12 |
Granzyme B | 0.989 | 20.0-4,850 | 1:2 | 1:2 | 63 |
Haptoglobin | 118 | 2,131-517,720 | 1:2 | 1:4,000 | 30 |
HGF | 3.33 | 39.6-9,620 | 1:2 | 1:2 | 12 |
ICAM-1 | 52.7 | 627-152,270 | 1:2 | 1:2 | 35 |
IFN-gamma | 1.85 | 12.3-2,990 | 1:2 | 1:2 | 33 |
IGF-I | 3.46 | 46.0-11,180 | 1:2 | 1:50 | 55 |
IGFBP-1 | 0.218 | 1.85-450 | 1:2 | 1:200 | 33 |
IGFBP-3 | 7.46 | 53.1-12,900 | 1:2 | 1:4,000 | 65 |
IL-1 alpha | 8.17 | 28.6-6,960 | 1:2 | 1:2 | 47 |
IL-1 beta | 41.8 | 221-53,780 | 1:2 | 1:2 | 19 |
IL-2 | 0.481 | 7.7-1,860 | 1:2 | 1:2 | 22 |
IL-3 | 0.200 | 2.63-640 | 1:2 | 1:2 | 34 |
IL-4 | 53.0 | 56.4-13,700 | 1:2 | 1:2 | 25 |
IL-5 | 0.237 | 4.7-1,130 | 1:2 | 1:2 | 26 |
IL-6 | 2.30 | 24.6-5,970 | 1:2 | 1:2 | 27 |
IL-6 R alpha | 26.8 | 431-104,770 | 1:2 | 1:2 | 18 |
IL-7 | 35.4 | 267-64,770 | 1:2 | 1:2 | 14 |
IL-10 | 8.20 | 11.7-2,840 | 1:2 | 1:2 | 28 |
IL-12 p70 | 12.8 | 42.1-10,240 | 1:2 | 1:2 | 15 |
IL-13 | 6.46 | 59.2-14,390 | 1:2 | 1:2 | 29 |
IL-16 | 0.547 | 14.2-3,460 | 1:2 | 1:2 | 35 |
IL-17A | 7.08 | 56.2-13,660 | 1:2 | 1:2 | 30 |
IL-17E/IL-25 | 8.88 | 147-35,790 | 1:2 | 1:2 | 55 |
IL-23 p19 | 47.7 | 108-26,150 | 1:2 | 1:2 | 42 |
IL-27 | 1.84 | 45.2-10,990 | 1:2 | 1:2 | 56 |
IL-33 | 57.1 | 79.1-19,220 | 1:2 | 1:2 | 43 |
LDL R | 411 | 1,227-298,080 | 1:2 | 1:2 | 37 |
Lipocalin-2/NGAL | 66.1 | 373-90,620 | 1:2 | 1:50 | 44 |
LIX | 2.02 | 18.1-4,410 | 1:2 | 1:2 | 66 |
M-CSF | 0.404 | 1.85-450 | 1:2 | 1:2 | 45 |
MMP-2 | 48.8 | 214-51,970 | 1:2 | 1:200 | 37 |
MMP-3 | 0.332 | 15.3-3,720 | 1:2 | 1:200 | 62 |
MMP-8 | 2,109 | 4,657-1,131,617 | 1:2 | 1:2 | 61 |
MMP-9 | 47.1 | 92.8-22,560 | 1:2 | 1:50 | 62 |
MMP-12 | 0.424 | 20.9-5,090 | 1:2 | 1:2 | 73 |
Nephrin | 154 | 680-165,220 | 1:2 | 1:2 | 38 |
Oncostatin M | 6.26 | 72.3-17,580 | 1:2 | 1:2 | 64 |
Osteopontin | 2.37 | 36.4-8,840 | 1:2 | 1:50 | 56 |
Osteoprotegerin | 6.32 | 48.9-11,890 | 1:2 | 1:2 | 39 |
Pancreatic Polypeptide | 23.3 | 252-61,160 | 1:2 | 1:2 | 45 |
PDGF-AA | 0.903 | 4.16-1,010 | 1:2 | 1:200/1:2 | 14 |
PDGF-AB | 0.725 | 8.20-1,993 | 1:2 | 1:2 | 46 |
PDGF-BB | 1.49 | 29.4-7,150 | 1:2 | 1:50 | 27 |
Periostin/OSF-2 | 201 | 1,370-332,890 | 1:2 | 1:200 | 67 |
PlGF-2 | 1.70 | 9.37-2,270 | 1:2 | 1:2 | 44 |
Podocalyxin | 5.03 | 115-28,000 | 1:2 | 1:2 | 77 |
P-Selectin | 2.60 | 138-33,580 | 1:2 | 1:2 | 46 |
Prolactin | 2.67 | 64.0-15,550 | 1:2 | 1:2 | 44 |
Proprotein Convertase 9/ PCSK9 | 289 | 430-104,500 | 1:2 | 1:2 | 53 |
RAGE | 18.0 | 54.3-13,200 | 1:2 | 1:2 | 78 |
Renin | 13.2 | 129-31,300 | 1:2 | 1:2 | 47 |
Resistin | 0.737 | 3.3-800 | 1:2 | 1:50 | 36 |
S100A8 | 61.3 | 278-67,490 | 1:2 | 1:2 | 20 |
S100A9 | 3.55 | 110-26,720 | 1:2 | 1:2 | 21 |
Serpin E1/PAI-1 | 5.71 | 30.2-7,340 | 1:2 | 1:50 | 43 |
SOST | 2.27 | 16.3-3,950 | 1:2 | 1:2 | 48 |
SP-D | 11.0 | 234-56,760 | 1:2 | 1:2 | 12 |
Syndecan-1/CD138 | 288 | 1,066-259,100 | 1:2 | 1:2 | 51 |
Thrombospondin-4 | 17.5 | 256-62,290 | 1:2 | 1:200 | 13 |
TIM-1/KIM-1 | 13.1 | 257-62,380 | 1:2 | 1:2 | 72 |
TIMP-1 | 12.4 | 59.1-14,350 | 1:2 | 1:2 | 45 |
TIMP-4 | 14.4 | 123-29,850 | 1:2 | 1:2 | 47 |
TNF-alpha | 1.47 | 3.7-900 | 1:2 | 1:2 | 14 |
TNF RI | 0.124 | 7.86-1,910 | 1:2 | 1:2 | 15 |
TNF RII | 2.48 | 46.7-11,340 | 1:2 | 1:2 | 74 |
TRANCE/RANK L | 7.51 | 59.1-14,370 | 1:2 | 1:2 | 25 |
TWEAK | 0.184 | 2.88-700 | 1:2 | 1:2 | 53 |
uPAR | 88.2 | 1,080-262,520 | 1:2 | 1:2 | 76 |
VEGF | 3.96 | 4.7-1,130 | 1:2 | 1:2 | 21 |
VEGF R2/KDR | 27.5 | 267-64,810 | 1:2 | 1:2 | 26 |
*A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay. |
Assays for the Luminex platform are offered as or . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit.
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