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Assay Type
Solid Phase Sandwich ELISA
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Format
96-well strip plate
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Assay Length
4.5 hours
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Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
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Sensitivity
15 pg/mL
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Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Specificity
Natural and recombinant human PDGF-BB
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Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
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Interference
No significant interference observed with available related molecules.
Control Available
QC22, Quantikine Immunoassay Control Group 7 -
Product Summary
The Quantikine Human PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-BB in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human PDGF-BB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-BB.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 268 | 639 | 935 | 251 | 655 | 918 |
| Standard Deviation | 11.3 | 28.4 | 26.8 | 22.4 | 38.5 | 59.3 |
| CV% | 4.2 | 4.4 | 2.9 | 8.9 | 5.9 | 6.5 |
Serum, EDTA Plasma, Heparin Plasma | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 310 | 759 | 1093 | 287 | 772 | 1082 |
| Standard Deviation | 14 | 18.6 | 25.5 | 21.9 | 56.9 | 89.8 |
| CV% | 4.5 | 2.4 | 2.3 | 7.6 | 7.4 | 8.3 |
Recovery
The recovery of PDGF-BB spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 94 | 86-102 |
| Platelet-poor EDTA Plasma (n=6) | 101 | 88-115 |
| Platelet-poor Heparin Plasma (n=6) | 103 | 89-112 |
| Serum (n=6) | 102 | 93-114 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of PDGF-BB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: PDGF-BB
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
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Alternate Names
PDGFBB; PDGF-BB;
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