Human IL-12/IL-23 p40 Quantikine SixPak (6 Plates) 1 PK

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Human IL-12/IL-23 p40 Quantikine SixPak (6 Plates) 1 PK

市场价：: 40460.00

促销价：: ¥40460.00

货号：SP400

品牌：R&D Systems

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计7-14工作日到货(以R&D Systems官网货期为准))

工作时间

周一至周五：9:00-18:00

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0771-3293894

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商品介绍
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Assay Type

Solid Phase Sandwich ELISA
Format

96-well strip plate
Assay Length

3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity

15 pg/mL
Assay Range

31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity

Natural and recombinant human IL-12 p40
Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference

No significant interference observed with available related molecules.

Control Available

QC22, Quantikine Immunoassay Control Group 7 -

Product Summary

The Quantikine Human IL-12 p40 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-12 p40 in cell culture supernates, serum, and plasma. It contains recombinant human IL-12 p40 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-12 p40. Results obtained using natural human IL-12 p40 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12 p40.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	151	380	1138	160	399	1145
Standard Deviation	9.9	27.2	62.3	11	22.3	63
CV%	6.6	7.2	5.5	6.9	5.6	5.5

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	119	290	871	127	315	891
Standard Deviation	5.1	11.4	35.5	11.8	19.8	60.6
CV%	4.3	3.9	4.1	9.3	6.3	6.8

Recovery

The recovery of IL-12 p40 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	96	85-105
Citrate Plasma (n=5)	100	85-112
EDTA Plasma (n=5)	101	92-113
Heparin Plasma (n=5)	96	89-106
Serum (n=5)	98	86-112

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of IL-12 p40 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human IL-12/IL-23 p40 Quantikine ELISA Kit

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12/IL-23 p40

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.