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Assay Type
Solid Phase Sandwich ELISA
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Format
96-well strip plate
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Assay Length
4.5 hours
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Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (75 uL), EDTA Plasma (75 uL), Heparin Plasma (75 uL), Saliva (10 uL), Urine (100 uL)
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Sensitivity
4.46 pg/mL
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Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
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Specificity
Natural and recombinant human IP-10
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Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
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Interference
No significant interference observed with available related molecules.
Control Available
QC23, Quantikine Immunoassay Control Group 8 -
Product Summary
The Quantikine Human IP-10 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IP-10 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IP-10 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IP-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IP-10.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma | Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean | 67.4 | 168 | 334 | 70.7 | 182 | 366 |
Standard Deviation | 3.12 | 4.99 | 11.15 | 6.23 | 11.12 | 18.93 |
CV% | 4.6 | 3 | 3.3 | 8.8 | 6.1 | 5.2 |
Cell Culture Supernates, Saliva, Urine | Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean | 70.3 | 174 | 342 | 80.7 | 194 | 362 |
Standard Deviation | 2.07 | 5.15 | 10.74 | 7.91 | 13.48 | 24.27 |
CV% | 2.9 | 3 | 3.1 | 9.8 | 6.9 | 6.7 |
Recovery
The recovery of IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
Cell Culture Media (n=4) | 95 | 88-101 |
EDTA Plasma (n=5) | 99 | 90-109 |
Heparin Plasma (n=5) | 99 | 87-113 |
Serum (n=5) | 99 | 88-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IP-10 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: CXCL10/IP-10/CRG-2
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.