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Assay Type
Solid Phase Sandwich ELISA
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Format
96-well strip plate
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Assay Length
4.5 hours
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Sample Type & Volume Required Per Well
Cell Culture Supernates (75 uL), Serum (75 uL), EDTA Plasma (75 uL), Heparin Plasma (75 uL), Urine (75 uL)
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Sensitivity
0.207 pg/mL
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Assay Range
0.4 - 25 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Specificity
Synthetic and natural Endothelin-1
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Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
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Interference
No significant interference observed with available related molecules.
Control Available
QC82, Quantikine Immunoassay Control Set 907 for Endothelin-1 -
Product Summary
The Quantikine Endothelin-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure ET-1 in cell culture supernates, serum, plasma, and urine. It contains synthetic ET-1 and antibodies raised against synthetic ET-1. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring ET-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 3 | 7.34 | 14.7 | 3.05 | 7.43 | 14.4 |
| Standard Deviation | 0.12 | 0.17 | 0.28 | 0.231 | 0.438 | 0.759 |
| CV% | 4 | 2.3 | 1.9 | 7.6 | 5.9 | 5.3 |
Recovery
The recovery of Endothelin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 99 | 88-108 |
| Human EDTA Plasma (n=4) | 93 | 87-102 |
| Human Heparin Plasma (n=4) | 93 | 85-106 |
| Human Serum (n=4) | 98 | 86-107 |
| Mouse EDTA Plasma (n=4) | 99 | 102-107 |
| Mouse Serum (n=4) | 94 | 86-104 |
| Rat EDTA Plasma (n=4) | 99 | 86-113 |
| Rat Serum (n=4) | 98 | 88-113 |
| Urine (n=4) | 91 | 85-109 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Endothelin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: Endothelin-1
Endothelin-1 (ET-1) is produced by many tissues including lung, kidney, brain, endocrine glands, and placenta. It is also expressed by several cell types including, mast cells, endothelial cells, epithelial and smooth muscle cells. The most prominent source is vascular endothelium. ET-1 is best known as a potent vasoconstrictor. Elevated blood ET-1 levels are associated with a variety of diseases, possibly as a function of a stress response.
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