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Assay Type
Solid Phase Sandwich ELISA
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Format
96-well strip plate
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Assay Length
4.5 hours
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Sample Type & Volume Required Per Well
Cell Lysates (20 uL), Subcellular Fractions (100 uL)
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Sensitivity
0.31 ng/mL
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Assay Range
0.6 - 20 ng/mL (Cell Lysates, Subcellular Fractions)
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Specificity
Natural human Cytochrome c
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Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
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Interference
Interference observed with other substances.
Control Available
QC75, Quantikine Immunoassay Control Set 795 Human Cytochrome c -
Product Summary
The Quantikine Human Cytochrome c Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human cytochrome c in cell lysates.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Lysates, Subcellular Fractions | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 3.64 | 7.31 | 10.7 | 3.54 | 6.74 | 10.2 |
| Standard Deviation | 0.15 | 0.3 | 0.42 | 0.18 | 0.34 | 0.51 |
| CV% | 4.1 | 4.1 | 3.9 | 5.1 | 5 | 5 |
Linearity
To assess the linearity of the assay, six cell lysates containing high concentrations of Cytochrome c were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: Cytochrome c
Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.
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Entrez Gene IDs
54205 (Human);
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