-
Assay Type
Solid Phase Sandwich ELISA
-
Format
96-well strip plate
-
Assay Length
2.5 hours
-
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
-
Sensitivity
0.053 ng/mL
-
Assay Range
0.6 - 40 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
-
Specificity
Natural and recombinant human ICAM-1
-
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
-
Interference
No significant interference observed with available related molecules.
Control Available
QC236, Quantikine Immunoassay Control Group 236 -
Product Summary
The Quantikine Human ICAM-1/CD54 Non Allele-specific Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human ICAM-1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human ICAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ICAM-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 3.03 | 9.64 | 20 | 3.27 | 9.81 | 19.5 |
| Standard Deviation | 0.128 | 0.346 | 0.973 | 0.282 | 0.543 | 1.22 |
| CV% | 4.2 | 3.6 | 4.9 | 8.6 | 5.5 | 6.3 |
Recovery
The recovery of human ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 103 | 96-117 |
| Urine (n=4) | 94 | 85-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ICAM-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: ICAM-1/CD54
ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.
新浪微博
腾讯微博
QQ空间
客服
