Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 100 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples in the polystyrene bead format. Up to 50 analytes can be screened in the magnetic bead format.
Analyte | Sensitivity (MDD)pg/mL | Standard Curve Range pg/mL* | Recommended Starting Dilution | Bead Region | |||
---|---|---|---|---|---|---|---|
Cell Culture Supernates | Serum | EDTA Plasma | Heparin Plasma | ||||
CXCL2/CINC-3 | 10.4 | 28.1-6,830 | 1:2 | 1:2 | 1:2 | 1:2 | 13 |
CXCL3/CINC-2 alpha/beta | 3.10 | 23.2-5,640 | 1:2 | 1:2 | 1:2 | 1:2 | 12 |
GM-CSF | 7.91 | 42.2-10,260 | 1:2 | 1:2 | 1:2 | 1:2 | 14 |
ICAM-1/CD54 | 7.52 | 35.9-8,720 | 1:2 | 1:2 | 1:2 | 1:2 | 15 |
IFN-gamma | 70.5 | 509-123,600 | 1:2 | 1:2 | 1:2 | 1:2 | 18 |
IL-1 alpha/ IL-1F1 | 13.0 | 72.0-17,500 | 1:2 | 1:2 | 1:2 | 1:2 | 19 |
IL-1 beta/ IL-1F2 | 26.7 | 18.5-4,500 | 1:2 | 1:2 | 1:2 | 1:2 | 20 |
IL-2 | 45.9 | 21.9-5,330 | 1:2 | 1:2 | 1:2 | 1:2 | 21 |
IL-4 | 5.41 | 13.2-3,210 | 1:2 | 1:2 | 1:2 | 1:2 | 22 |
IL-6 | 56.0 | 170-41,300 | 1:2 | 1:2 | 1:2 | 1:2 | 25 |
IL-10 | 18.6 | 19.7-4,790 | 1:2 | 1:2 | 1:2 | 1:2 | 26 |
IL-13 | 6.08 | 5.19-1,260 | 1:2 | 1:2 | 1:2 | 1:2 | 27 |
IL-18 | 8.89 | 14.2-3,440 | 1:2 | 1:2 | 1:2 | 1:2 | 28 |
L-Selectin/ CD62L | 33.6 | 86.2-20,950 | 1:2 | 1:2 | 1:2 | 1:2 | 29 |
TIMP-1 | 5.38 | 26.1-6,340 | 1:2 | 1:2 | 1:2 | 1:2 | 30 |
TNF-alpha | 22.1 | 232-56,400 | 1:2 | 1:2 | 1:2 | 1:2 | 33 |
VEGF | 4.28 | 62.1-15,100 | 1:2 | 1:2 | 1:2 | 1:2 | 34 |
NR=Not Recommended
MDD=Minimum Detectable Dose
*A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Assays for the Luminex platform are offered as or . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit.
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