Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 23 | 23 | 23 |
Mean | 0.33 | 1.23 | 3.83 | 0.35 | 1.22 | 4.02 |
Standard Deviation | 0.022 | 0.072 | 0.221 | 0.021 | 0.061 | 0.258 |
CV% | 6.7 | 5.9 | 5.8 | 6 | 5 | 6.4 |
The recovery of mouse Adiponectin spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Supernates (n=7) | 103 | 82-115 |
EDTA Plasma (n=4) | 92 | 81-106 |
Heparin Plasma (n=4) | 93 | 77-110 |
Serum (n=6) | 98 | 85-114 |
Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.
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