-
Assay Type
Solid Phase Sandwich ELISA
-
Format
96-well strip plate
-
Assay Length
3.5 hours or 4.5 hours
-
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
-
Sensitivity
1 pg/mL
-
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
-
Specificity
Natural and recombinant human IL-1 beta
-
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
-
Interference
Interference observed with 1 or more available related molecules.
Control Available
QC01-1, Quantikine Immunoassay Control Group 1 -
Product Summary
The Quantikine Human IL-1 beta Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-1 beta accurately. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.
Precision
Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 18.9 | 30.2 | 122 | 181 | 29.8 | 118 | 193 |
| Standard Deviation | 1.6 | 1 | 5.4 | 5 | 2.5 | 5 | 8 |
| CV% | 8.5 | 3.3 | 4.4 | 2.8 | 8.4 | 4.2 | 4.1 |
Cell Culture Supernates | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 12.6 | 30.4 | 69.2 | 192 | 30.8 | 120 | 194 |
| Standard Deviation | 0.3 | 1 | 1.6 | 6.5 | 2.2 | 4.1 | 7.9 |
| CV% | 2.4 | 3.3 | 2.3 | 3.4 | 7.1 | 3.4 | 4.1 |
Recovery
The recovery of IL-1 beta spiked to different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 97 | 80-111 |
| Citrate Plasma (n=10) | 93 | 83-110 |
| EDTA Plasma (n=10) | 86 | 81-100 |
| Heparin Plasma (n=10) | 82 | 76-100 |
| Serum (n=10) | 95 | 87-110 |
Linearity
To assess linearity of the assay, the following biological samples containing, or spiked with, high concentrations of IL-1 beta were diluted with the appropriate Calibrator Diluent and then assayed.
Preparation and Storage
Background: IL-1 beta/IL-1F2
IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.
-
Long Name
Interleukin 1 beta
新浪微博
腾讯微博
QQ空间
客服
