-
Assay Type
Solid Phase Sandwich ELISA
-
Format
96-well strip plate
-
Assay Length
3.5 hours or 4.5 hours
-
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
-
Sensitivity
1 pg/mL
-
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
-
Specificity
Natural and recombinant human IL-1 alpha
-
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
-
Interference
Interference observed with 1 or more available related molecules.
Control Available
QC01-1, Quantikine Immunoassay Control Group 1 -
Product Summary
The Quantikine Human IL-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 alpha in cell culture supernates, serum, and plasma. It contains recombinant human IL-1 alpha and antibodies raised against recombinant human IL-1 alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 alpha showed linear curves that were parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1 alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 28.6 | 125 | 185 | 27.9 | 123 | 187 |
| Standard Deviation | 0.4 | 1.4 | 4 | 1.2 | 4.7 | 6.3 |
| CV% | 1.4 | 1.1 | 2.2 | 4.3 | 3.8 | 3.4 |
Cell Culture Supernates | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 26.9 | 122 | 191 | 20.5 | 60 | 121 |
| Standard Deviation | 0.4 | 4.6 | 6.6 | 1.7 | 3.1 | 5.2 |
| CV% | 1.5 | 3.8 | 3.5 | 8.3 | 5.2 | 4.3 |
Recovery
The recovery of IL-1 alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 96 | 91-104 |
| Citrate Plasma (n=6) | 97 | 71-119 |
| EDTA Plasma (n=6) | 104 | 84-121 |
| Heparin Plasma (n=6) | 104 | 72-126 |
| Serum (n=6) | 107 | 74-127 |
Linearity
To assess the linearity of the assay, the following biological samples spiked with high concentrations of IL-1 alpha were diluted with the appropriate Calibrator Diluent and then assayed.
Preparation and Storage
Background: IL-1 alpha/IL-1F1
IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.
-
Long Name
Interleukin 1 alpha
新浪微博
腾讯微博
QQ空间
客服
