-
Assay Type
Solid Phase Sandwich ELISA
-
Format
96-well strip plate
-
Assay Length
4.5 hours
-
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
-
Sensitivity
0.022 ng/mL
-
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
-
Specificity
Natural and recombinant human CRP
-
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
-
Interference
No significant interference observed with available related molecules.
Control Available
QC70, Quantikine Immunoassay Control Set 960 for Human CRP -
Product Summary
The Quantikine Human CRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CRP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CRP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CRP.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma | Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean | 4.79 | 8.66 | 18.9 | 4.84 | 8.66 | 17.5 |
Standard Deviation | 0.21 | 0.33 | 1.57 | 0.29 | 0.61 | 1.15 |
CV% | 4.4 | 3.8 | 8.3 | 6 | 7 | 6.6 |
Recovery
The recovery of CRP spiked to levels throughout the range of the assay in cell culture media was evaluated.
Sample Type | Average % Recovery | Range % |
Cell Culture Media (n=4) | 100 | 92-110 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CRP were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: C-Reactive Protein/CRP
C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.