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For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.
| Analyte | Catalog Number | Sensitivity (pg/mL) | High Standard Value* (pg/mL) | Microparticle Region |
|---|---|---|---|---|
| BAFF/BLyS/TNFSF13B | 0.56 | 2530 | 13 | |
| CCL20/MIP-2 alpha | 0.11 | 1,890 | 19 | |
| CD14 | 20 | 141,300 | 14 | |
| CD25/IL-2 R alpha | 0.45 | 7,650 | 20 | |
| CD27/TNFRSF7 | 3.23 | 14,900 | 34 | |
| CXCL13/BLC/BCA-1 | LBAM801 | 0.56 | 2,120 | 36 |
| Gp130 | 12 | 89,440 | 15 | |
| IL-6 R alpha | 1.01 | 12,100 | 30 | |
| TNF RII | LUCAM726 | 0.2 | 870 | 25 |
| *A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. | ||||
| Analyte | Cell Culture Supernate | Serum | EDTA Plasma | Heparin Plasma | |
|---|---|---|---|---|---|
| BAFF/BLyS/TNFSF13B | 1:2 | 1:3 | 1:3 | 1:3 | |
| CCL20/MIP-2 alpha | 1:2 | 1:3 | 1:3 | 1:3 | |
| CD14 | 1:2 | 1:50 | 1:50 | 1:50 | |
| CD25/IL-2 R alpha | 1:2 | 1:3 | 1:3 | 1:3 | |
| CD27/TNFRSF7 | 1:2 | 1:3 | 1:3 | 1:3 | |
| CXCL13/BLC/BCA-1 | 1:2 | 1:3 | 1:3 | 1:3 | |
| Gp130 | 1:2 | 1:50 | 1:50 | 1:50 | |
| IL-6 R alpha | 1:2 | 1:50 | 1:50 | 1:50 | |
| TNF RII | 1:2 | 1:50 | 1:50 | 1:50 | |
| N/R=This sample type is not recommended for use in this assay | |||||
| *CD40 Ligand is present in platelet granules and is released upon platelet activation. Therefore, to measure circulating levels of CD40 Ligand, platelet-free plasma should be collected for measurement. | |||||
Magnetic are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
Assays for the Luminex platform are offered as or . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. .
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