Human HS Cytokine A Premixed Mag Luminex Performance Assay 7 PLEX

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Human HS Cytokine A Premixed Mag Luminex Performance Assay 7 PLEX

市场价：: 23973.00

促销价：: ¥23973.00

货号：FCSTM09-07

品牌：R&D Systems

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计7-14工作日到货(以R&D Systems官网货期为准))

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

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: 客服郭恒蔡玉坤曾宪飞技术咨询

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商品介绍
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Assay Type

Magnetic bead-based multiplex assay for the Luminex ^® MAGPIX ^® platform
Format

96-well microplate and antibody-coated magnetic beads*
Analytes Detected

Please see table below.
Performance Validation

For standard curves, intra-assay and inter-assay precision, recovery, and linearity data for the different analyte-specific beads, please see product datasheets for corresponding individual Bead Sets (below).

Analytes

Select analytes you would like to measure from the list below. Please note that analytes with the same bead region are not compatible in the same assay and when selected will populate a new assay after clicking the "continue" button.

** Initial Starting Dilutions

Select Your Analytes below or Search here:

	Analyte	Standard Curve (pg/mL)	Cell Culture Supernates	Serum	Plasma

* Note: This analyte is in more than one assay. Please refer to the Technical Datasheet for information specific to this analyte.

* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).

Product Features

Simultaneously detect multiple analytes
Magnetic format allows for easier wash steps
User selects specific analytes of interest
Requires small sample size
Undergoes similar validation testing as Quantikine^® ELISA Kits
Precise and Specific

Base Kit Contents

Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent(s)
Streptavidin-PE
Wash Buffer Concentrate
Mixing Bottles
Round-bottom Microplate
Foil Plate Sealers

Bead Set Contents

Antibody-coated magnetic beads
Biotinylated detection antibody

Information for Compatiable Analytes

For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.

This kit is validated for human serum, EDTA plasma, and heparin plasma samples. All samples have a recommended starting dilution of 1:2.

Analyte	Catalog Number	Sensitivity (pg/mL)	High Standard Value* (pg/mL)	Microparticle Region
GM-CSF		0.13	1,600	29
IFN-gamma		0.25	22,400	30
IL-1 beta/IL-1F2		0.18	1,200	20
IL-2		0.28	2,500	19
IL-4		2.54	7,200	21
IL-5		0.12	1,400	22
IL-6		0.31	3,900	25
CXCL8/IL-8		0.07	3,200	26
IL-10		0.24	1,900	27
IL-12 p70		2.96	28,300	28
TNF-alpha		0.54	3,100	12
VEGF		1.35	2,300	13
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

More Information about Magnetic Bead-Based Multiple Kits for the Luminex Platform

Magnetic multiplex kits are designed for use with the CCD Imager. Alternatively, kits can be used with the or Bio-Rad^® Bio-Plex^®, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded magnetic microparticles. Microparticles, standards and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated antibody, is added to each well. A final wash removes unbound Streptavidin-PE, the microparticles are resuspended in buffer and read using the Luminex MAGPIX Analyzer. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

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