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Format
96-well strip plate
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Assay Length
3.5 hours
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Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL)
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Sensitivity
43.8 pg/mL
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Assay Range
39.0 - 2,500 pg/mL (Cell Culture Supernates)
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Specificity
Measures Substance P levels in various samples
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Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
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Interference
No significant interference observed with available related molecules.
Control Available
QC163, Parameter Immunoassay ControlSet 657 for Substance P -
Product Summary
The Parameter Substance P Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure Substance P in cell culture supernates, serum, plasma, saliva, and urine. It contains a synthetically derived human Substance P peptide and has been shown to accurately quantitate this peptide. Results obtained using samples containing natural Substance P showed linear curves that were parallel to the standard curves obtained using the Parameter kit standards. These results indicate that this kit can be used to determine relative mass values for natural Substance P.
Precision
Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
| n | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 146 | 258 | 616 | 1973 | 140 | 262 | 684 | 1923 |
| Standard Deviation | 12.3 | 15.1 | 21.5 | 142 | 18.1 | 26.8 | 103 | 180 |
| CV% | 8.4 | 5.8 | 3.5 | 7.2 | 12.9 | 10.2 | 15 | 9.3 |
Recovery
The recovery of Substance P spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 94 | 82-110 |
| Heparin Plasma (n=4) | 100 | 85-116 |
| Saliva (n=4) | 102 | 90-110 |
| Serum (n=4) | 101 | 85-117 |
| Urine (n=4) | 89 | 76-107 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Substance P were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: Substance P
Substance P (SP, Neurokinin-1), is member of the Tachykinin peptide family and binds primarily to the Neurokinin 1 Receptor (NK1R, Substance P receptor, Tachykinin receptor 1) and with low affinity to the NK2 and NK3 receptors. Substance P is processed from preproTachykinin a, b, g and d isoforms that also generate other Tachykinins Neuropeptide K, Neurokinin A, Neurokinin B, and Neuropeptide G. Substance P is produced in the central nervous system, primary sensory neurons (C-type fibers), and endocrine cells of the intestinal mucosa. It triggers pain signals in the spinal cord dorsal horn as well as hypotension, tachycardia, release of inflammatory mediators, and direct antimicrobial action.
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Alternate Names
neurokinin 1; neurokinin 2; neurokinin A; Neurokinin-1; neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma); neuropeptide gamma; neuropeptide K; NK2; NKA; NKNAneurokinin alpha; NPK; PPT; protachykinin-1; substance K; Substance P; TAC2neuromedin L; tachykinin 2; tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2; tachykinin, precursor 1;
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