For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.
Analyte | Catalog Number | Sensitivity (pg/mL) | High Standard Value* (pg/mL) | Microparticle Region |
---|---|---|---|---|
Adiponectin/Acrp30 | 6.4 | 340,000 | 46 | |
C-Reactive Protein | 1.4 | 14,800 | 48 | |
CCL2/MCP-1 | 0.16 | 2,100 | 33 | |
Complement Factor D/Adipsin | 1.8 | 91,500 | 47 | |
IL-6 | 0.36 | 4,950 | 27 | |
IL-10 | 0.13 | 3,200 | 29 | |
Leptin | 7.69 | 36,500 | 45 | |
Resistin | 0.85 | 33,200 | 56 | |
Serpin E1/PAI-1 | 0.2 | 10,300 | 26 | |
TNF-alpha | 0.6 | 4,500 | 36 | |
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. |
Analyte | Cell Culture Supernate | Serum | EDTA Plasma | Heparin Plasma | |
---|---|---|---|---|---|
Adiponectin/Acrp30 | None | 1:100 | 1:100 | 1:100 | |
C-Reactive Protein | None | 1:100 | 1:100 | 1:100 | |
CCL2/MCP-1 | None | 1:4 | 1:4 | 1:4 | |
Complement Factor D/Adipsin | None | 1:100 | 1:100 | 1:100 | |
IL-6 | None | 1:4 | 1:4 | 1:4 | |
IL-10 | None | 1:4 | 1:4 | 1:4 | |
Leptin | None | 1:4 | 1:4 | 1:4 | |
Resistin | None | 1:4 | 1:4 | 1:4 | |
Serpin E1/PAI-1 | None | 1:100 | 1:100 | 1:100 | |
TNF-alpha | None | 1:4 | 1:4 | 1:4 | |
N/R=This sample type is not recommended for use in this assay | |||||
*CD40 Ligand is present in platelet granules and is released upon platelet activation. Therefore, to measure circulating levels of CD40 Ligand, platelet-free plasma should be collected for measurement. |
Magnetic are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
Assays for the Luminex platform are offered as or . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. .
Trademarks and registered trademarks are the property of their respective company.
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894