* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).

• Simultaneously detect multiple analytes
• Magnetic format allows for easier wash steps
• User selects specific analytes of interest
• Requires small sample size
• Undergoes similar validation testing as Quantikine^® ELISA Kits
• Precise and Specific

• Standard Cocktail(s)
• Bead Diluent
• Biotin Antibody Diluent
• Calibrator Diluent(s)
• Streptavidin-PE
• Wash Buffer Concentrate
• Mixing Bottles
• Round-bottom Microplate
• Foil Plate Sealers

• Antibody-coated magnetic beads
• Biotinylated detection antibody

Magnetic multiplex kits are designed for use with the CCD Imager. Alternatively, kits can be used with the or Bio-Rad^® Bio-Plex^®, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded magnetic microparticles. Microparticles, standards and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated antibody, is added to each well. A final wash removes unbound Streptavidin-PE, the microparticles are resuspended in buffer and read using the Luminex MAGPIX Analyzer. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

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